Construction of a binary vector for knockout and expression analysis of rice blast fungus genes.

نویسندگان

  • Ken-ichiro Saitoh
  • Marie Nishimura
  • Yasuyuki Kubo
  • Nagao Hayashi
  • Eiichi Minami
  • Yoko Nishizawa
چکیده

We developed an efficient method to analyze gene function and expression of the rice blast fungus. We constructed a GATEWAY binary vector, which generates a gene-targeted disruptant carrying a green fluorescent protein gene under the native promoter of the target gene. Using this method, the knockout efficiency and expression patterns of two hypothetical genes were determined.

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عنوان ژورنال:
  • Bioscience, biotechnology, and biochemistry

دوره 72 5  شماره 

صفحات  -

تاریخ انتشار 2008